Alright, let’s talk about this cell thingy, you know, the one they grow in them little dishes. What’s the least amount of juice you gotta put in there? That’s what they’re askin’, right? “Minimum volume,” they call it. Sounds fancy, but it ain’t rocket science, I tell ya.
Now, I ain’t no scientist, but I heard them talking. It’s like plantin’ seeds, see? You can’t just sprinkle a few drops of water and expect a whole garden. Them cells, they need enough food, or whatever they eat, to swim around and grow big and strong. They call that “medium,” like it’s somethin’ special, but it’s just cell food, far as I can tell.
So, how much of this food you need? Well, it depends, like everything else in life. It’s like bakin’ a cake. A tiny cupcake don’t need as much batter as a big ol’ sheet cake, right? Same goes for these cell dishes. Them little bitty ones, they don’t need much. Them bigger ones, they need more, simple as that.
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I heard someone say you need somethin’ like 0.2 or 0.3 milliliters of that food for every little square thingy on the dish. They call it a “centimeter,” but it’s just a way of measurin’, like inches or feet. So, if you got a dish with, say, 10 of them little squares, you need 2 or 3 milliliters of food. Makes sense, don’t it?
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And if you got one of them fancy bottles, the ones they call “reservoirs,” well, you gotta have enough so the food can slosh around and mix good. They say 1 or 2 milliliters at least, otherwise it’s like tryin’ to stir soup in a thimble.
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But listen, there’s more. Them little plates with all them tiny holes, the ones they use for testin’ lots of stuff at once? Don’t go puttin’ just a drop in each hole. You gotta have enough so you can actually measure it, you know? Someone said not less than 100 microliters, which is like a teeny tiny bit, but still, it’s gotta be enough.
And then there’s the flasks, the ones that look like little bottles. Them T75 ones, they hold about 10 milliliters all together. The T25 ones, they’re smaller, hold about 5. And the P100 plates, they hold 8. You gotta put enough food in there to keep them cells happy, but not so much that they drown, I guess.
Now, someone was talkin’ about changin’ the food, too. Said you gotta do it after a few hours or the next day, to get rid of the old stuff. Makes sense, I guess. You wouldn’t eat leftovers all week, would ya? Same goes for them cells. And you need to put the right amount back in, so you got enough space for the cells to do whatever it is they do.
It’s all about gettin’ the right mix, see? Not too much, not too little, just right. Like Goldilocks and the three bears, only with cell food instead of porridge. You want them cells to grow up big and strong, so you gotta give them enough to eat and enough space to swim around. It ain’t rocket science, like I said. Just common sense, far as I can figure.
So, if you’re askin’ me what the least amount of juice is, well, it depends on the size of your dish or bottle or whatever. But remember, more is better than less, up to a point, I reckon. You don’t want them cells starvin’, that’s for sure. Just use your head, and you’ll figure it out.
And one more thing, them fancy bottles, they got this special somethin’ in ’em that helps the liquid flow better. Somethin’ about “low dead volume,” they call it. Means you don’t waste as much, I guess. Smart folks, them scientists, always thinkin’ of somethin’ new.
Anyways, that’s the gist of it. Keep them cells fed and watered, and they’ll do their thing. And don’t skimp on the food, that’s the main thing. Just like feedin’ chickens, you gotta give ’em enough to grow big and strong.
Tags: Cell Culture, Minimum Volume, Cell Growth, Cell Culture Reservoir, Cell Culture Dishes, Cell Medium, Cell Feeding
